Add Publication

Silkworm model for Francisella novicida infection

Publication Date - 2017-10-21 00:00:00

Publication Title : Silkworm model for Francisella novicida infection

Publicationed By : Shib Shankar Saha

Publication Publication Date : 2017-10-21 00:00:00

Publication Online Link :

Publication Description :

Understanding the virulence and pathogenesis of human pathogens using insect models is an increasingly  popular method. Francisella novicida, which is virulent in mice but non-pathogenic to immunocompetent humans,  is widely used as an ideal candidate for Francisella research. In this study, we developed a silkworm  (Bombyx mori) infection model for F. novicida by inoculating the hemocoels of silkworms with F. novicida. We  found that silkworms died within 3–7 days of F. novicida infection. However, the deletion mutant of DotU, the  core part of type VI secretion systems, failed to kill silkworm. In whole silkworm bodies, the bacterial load of the  DotU deletion mutant was significantly less than that of the wild-type strain. Approximately 10-fold increase in  bacterial load was recorded in hemolymph and subcutaneous tissues compared with that in the silk gland,  Malpighian tubule, and reproductive organs. The CFU count of the DotU deletion mutant in all organs was  similar results to the whole body CFU count. Confocal microscopy further confirmed the arrested growth of the  mutant strain within hemocytes. The intracellular growth of F. novicida strains was also analyzed using the  silkworm ovary-derived cell line BmN4. In BmN4, both CFU count assay and confocal microscopy revealed  extensive growth of the wild-type strain compared with that of the mutant strain. Francisella DotU has already  been proven as a virulence factor in mammals, and it was also found to be an essential virulence factor in our  silkworm infection model. Therefore, this silkworm infection model is suitable for identifying new virulence  factors of Francisella.

Recent Publications

A compact twofold spoon-shaped antenna for ultra-wideband (UWB) applications
W-shaped slot-loaded U-shaped low SAR patch antenna for microwave-based malignant tissue detection system
Current Status and Correlation of Fossil Fuels Consumption and Greenhouse Gas Emissions
Non-Financial and Financial Factors Influencing the Mode of Life of the Gher Farmers from the Western Coastal Areas of Bangladesh
Construction of a genetic linkage and detection of candidate genes of growth-related traits in pacific abalone
First record of whitespot sandsmelt, Parapercis alboguttata from the southeast coast of Bangladesh.
Effective accumulative temperature affects gonadal maturation in Pacific abalone, Haliotis discus hannai 
Thermal stress affects gonadal maturation by regulating GnRH, GnRH receptor, APGWamide, and serotonin receptor gene expression in male pacific abalone
Effects of Antifreeze protein III on sperm cryopreservation of Pacific abalone, Haliotis discus hannai
Carbonic anhydrase II (CA II) and its potential involvement in regulating shell formation in the Pacific abalone